Frequent Expression Tumor of the c・kit Proto - Oncogenein Canine Malignant Mammary
نویسنده
چکیده
,iBsTRAcr. The rnammary tumor is ene ot' the pepular nCop]astic diseascs in female degs. In the present sLudy, the exp[ession ofcftnine ckii proto-onceE,ene in mamrnary tumor specimens was invesligated to evalua{e its petential usefulness as a tumor maTker. By comparing thc homology amvng the nuc]eotide sequenees reported for human, mouse, rat und feline c-kit c-DNA, a pair of primcrs was synthesized for lhe reverse transcriptase-po]ymerase chain reaction (RT-PCR} mcthod, The RT-PCR product uf canine spleem total RNA was shown to have 756 bp in size und te be highly homologous Io thc corresponding sec]uences rcported for the mammalian spccies. The eNpressien
منابع مشابه
Frequent expression of the c-kit proto-oncogene in canine malignant mammary tumor.
The mammary tumor is one of the popular neoplastic diseases in female dogs. In the present study, the expression of canine c-kit proto-oncogene in mammary tumor specimens was investigated to evaluate its potential usefulness as a tumor marker. By comparing the homology among the nucleotide sequences reported for human mouse, rat and feline c-kit c-DNA, a pair of primers was synthesized for the ...
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BACKGROUND:Canine mammary gland cancers are the most prevalent malignancies in dogs. There are different challenges regarding management of these cancers in dogs and human, one hypothesis is related to small cellular subset of tumor mass called cancer stem cell. These cells are therapy resistant and cause metastasis and relapse even after primary successful treatment. The well-identified phenot...
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Background c-KIT is a proto-oncogene that synthesizes a tyrosine kinase protein responsible for cell growth. Higher expression of c-KIT protein in women normal breasts correlates with differentiation status of normal breast epithelium, while loss of c-KIT expression is observed during progression or malignant transformation of mammary epithelium. In bitches the role of this tyrosine kinase rece...
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In this study 80 malignant CMT were submitted to immunohistochemical detection of CD3, c-kit, VEGF, and CD31, together with clinicopathological parameters of tumor aggressiveness. CD3+ T-cells and c-kit overexpression revealed a positive correlation with VEGF (r = 0.503, P < 0.0001; r = 0.284, P = 0.023 for CD3 and c-kit, resp.) and CD31 (r = 0.654, P < 0.0001; r = 0.365, P = 0.003 for CD3 and ...
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